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KMID : 1001020070050030134
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Korean Journal of Urological Oncology
2007 Volume.5 No. 3 p.134 ~ p.137
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Newly Developed Effective Adenoviral Transportation Method through Selective Renal Arterial Catheterization in Rabbit
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Gil Myung-Cheol
Cho Jae-Wook
Hwang Jun-Seong
Yoon Seong-Kuk
Chung Jae-Il
Jung Se-Il
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Abstract
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Purpose: Many diseases originate from glomeruli of kidney. Proper delivery of genetic therapeutic materials into the glomeruli is a prerequisite for successful gene treatment in renal disease. Commonly used method of gene delivery to renal glomeruli in an animal model consists of injecting recombinant adenovirus through exposed abdominal aorta with abdominal incision. We developed a new method for recombinant adenovirus injection whereby renal glomeruli are approached through selective percutaneous carotid arterial catheterization in a rabbit model.
Materials and Methods : We produced recombinant adenovirus(Adv.CMV.LacZ) through human adenovirus type V without replication ability. The adenovirus is induced by cytomegalovirus(CMV) promoter and contains nuclear-targeted β-galactosidase gene. We inserted a catheter in the renal artery through selective percutaneous carotid arterial catheterization, and injected recombinant adenovirus as low(3×1012 particles/rabbit, n=6) or high(6×1012 particles/rabbit, n=6) dose under fluoroscopy for 2 hours. The rabbits were sacrificed three days after injection, and kidney, liver, spleen, intestine and lung were harvested and tested for LacZ expression.
Results: In rabbits injected with high dose, LacZ gene was expressed in 72±15% of the renal glomeruli(n=6), and was weakly expressed in renal tubular cells and endothelial cells. However, low dose injected rabbits did not express LacZ. The LacZ gene was expressed in 32±8% of hepatocytes, but not expressed in spleen, lung or intestinal tissues.
Conclusions : We successfully injected recombinant adenoviral vector in renal glomeruli through percutaneous selective renal catheterization. We propose this simple method of vector delivery without surgical procedure may be used in human gene therapy for renal diseases.
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KEYWORD
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Glomerulus, Adenovirus, Rabbit
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